《植物生理学报》 2018, 54(4): 618-626

丛枝菌根真菌摩西球囊霉对铜胁迫下白术幼苗光合特性及抗氧化酶活性的影响

杨国¹, 卢可¹, 朱高樑¹, 潘敏昕¹, 董丽佳¹, 罗洁^2,*, 莫亿伟^1,*

¹绍兴文理学院生命科学学院, 浙江绍兴312000; ²绍兴文理学院元培学院, 浙江绍兴312000

通信作者：罗洁；E-mail: lj26wlxy@163.com, ywmo@163.com

摘　要：

采用盆栽试验, 添加不同浓度(0、200、400 mg·kg^-1)铜(Cu), 研究接种丛枝菌根真菌(AMF)摩西球囊霉(Glomus mosseae)对白术(Atractylodes macrocephala)幼苗生长、光合特性、膜脂过氧化程度及抗氧化酶活性的影响。结果表明: 与对照相比, Cu胁迫使白术幼苗生长和叶片光合系统受到明显抑制, 膜脂过氧化水平升高, 抗氧化酶活性降低; 接种摩西球囊霉后发现其能够较好地侵染白术根系, 接种摩西球囊霉的白术幼苗在不同浓度Cu胁迫下, 均表现为叶片叶绿素a、叶绿素b和总叶绿素含量增加, 叶片净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)显著升高, 胞间CO₂浓度(C_i)显著下降, 质膜相对透性(MRP)及膜脂过氧化产物丙二醛(MDA)含量显著降低; 超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性较对照显著增加, 植株生长量和体内Cu积累量较对照均有所下降。以上结果说明接种摩西球囊霉可提高Cu胁迫下白术幼苗叶片光合能力和抗氧化能力, 降低膜脂过氧化水平, 减少白术根系对Cu的吸收, 缓解Cu胁迫对白术植株的伤害, 提高白术产量和品质。

关键词：丛枝菌根真菌; 摩西球囊霉; 白术; 铜胁迫; 光合特性; 抗氧化酶

收稿：2017-10-09   修定：2018-01-30

资助：国家自然科学基金(31700476)、绍兴市科技计划项目(2015B70023)、绍兴文理学院校级项目(2016LG1006)和浙江省大学生科技创新活动计划(新苗人才计划)项目(2017R428015)。

Effect of arbuscular mycorrhizal fungus Glomus mosseae on photosynthesis and antioxidation enzyme activities in Actractylodes macrocephala seedlings under copper stress

YANG Guo¹, Lu Ke¹, ZHU Gao-Liang¹, PAN Ming-Xin¹, DONG Li-Jia¹, LUO Jie^2,*, MO Yi-Wei^1,*

¹Academy of Life Science, Shaoxing University, Shaoxing, Zhejiang 312000, China; ²Yuanpei College, Shaoxing University, Shaoxing, Zhejiang 312000, China

Corresponding author: LUO Jie; E-mail: lj26wlxy@163.com, ywmo@163.com

Abstract:

Pot experiments were conducted to investigate effect of arbuscular mycorrhizal fungus (AMF) Glomus mosseae inoculations and copper ion (Cu) additions (0, 200 and 400 mg·kg^-1) on seedling growth, photosynthetic characteristics, lipid peroxidation and antioxidation enzyme activities in Actractylodes macrocephala. Results show that, compared to the control, Cu stress significantly inhibited growth and photosynthetic system of A. macrocephala, inhibited membrane lipid peroxidation and decreased activities of antioxidant enzymes. AMF colonized roots of A. macrocephala very well. For AMF inoculated seedlings, copper stress increased contents of chlorophyll a, chlorophyll b and total chlorophyll, net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r), and activities of superoxide dismutase (SOD), superoxide dismutase (POD) and catalase (CAT); decreased intercellular CO₂ concentration (C_i), relative permeability of plasma membrane (MRP) and malondialdehyde (MDA) content. In addition, plant biomass and Cu accumulation in plant were significantly decreased compared to the control. It was suggested that inoculation of AMF effectively alleviates damage of Cu stress on A. macrocephala by increasing photosynthetic ability and antioxidant capacity of A. macrocephala seedlings, and reducing membrane lipid peroxidation and Cu absorption by root system, thus adaptability to Cu stress and improved productivity and quality of A. macrocephala is being achieved.

Key words: arbuscular mycorrhizal fungus; Glomus mosseae; Actractylodes macrocephala; copper stress; photosynthetic characteristics; antioxidation enzyme